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Technologies

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We use the most appropriate technologies for each genetic study using our own or conventional procedures, according to the study needs

Seqplexing

EOSAL "Easy One-Step Amplification and Labelling" is a Sequencing Multiplex technology applicable to the detection of copy number variations (CNVs). This technology allows, in a single step, the amplification and labelling of each DNA fragment included in the reaction, and proportional to the amount of starting template. These amplicons are analyzed in a capillary sequencer, allowing detecting all the CNVs present in the regions analyzed. The whole process is done in less than 2.5 hours total and 10 minutes of hand-work. Our kits are designed for the minimum possible handling of reagents, samples and reaction products. This supposes a saving in the working time necessary for the development of a project and an important reduction in the possibility of errors and contaminations of the reactions and products

Our company also has extensive experience in numerous technologies, such as NGS from different types of samples, quantitative real-time PCR, capillary sequencing or analysis of fragments by capillary sequencer to perform studies of different types. In all cases, we simplify protocols to the maximum

List of technologies

Own technology

EOSAL "Easy One-Step Amplification and Labelling for CNV Detection"

Own technology alternative to MLPA

Seqplexing and INCLIVA have developed EOSAL "Easy One-Step Amplification and Labelling for CNV Detection" that has been protected by the corresponding patent. This technology allows, by means of a single amplification step, the obtaining of a large number of fragments labeled with the minimum cost and with a proportional amplification to the number of copies in the template of each of the amplified fragments

This technology has important advantages over the systems used for the same purposes, mainly due to the savings that they represent in time and reagents with great reliability (see figure). The entire process is performed in less than 2.5 hours total and less than 10 minutes of hand-work, analysis included. The time needed to perform a study is much less with EOSAL than that with the MLPA or other techniques for the detection of CNVs

We have kits to detect CNVs in the LDLR, BRCA1, BRCA2, LDLR, CHECK, MSH1, MLH2 and EPCAM genes, and we are developing new kits

EOSAL-CNV is applicable to virtually any gene or genetic region. Therefore, we can develop kits to analyze any region of the genome or disease where CNVs may be important. On the other hand, kits may include the simultaneous detection of CNVs and point mutations when their identification is important in a diagnosis or genetic study

Advantage

Our kits are designed for the minimum handling of reagents, samples and products (see figure EOSAL-CNV)

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Conventional technologies

In Seqplexing, we use different technologies to develop our kits or to carry out different kind of genetic studies. We are currently using the following systems:

Seqplexing

New generation sequencing (NGS) using the MiSeq System (Illumina)

Conventional technologies

Our company offers a sequencing service using the MiSeq system (Illumina). This service includes the verification of the library or "pool" and its quantification by fluorescence / quantitative PCR. We can perform standard, special and urgent sequencer runs with reduced delivery times (consult options and prices at info@seqplexing.com)

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Seqplexing

Omics studies using NGS

Conventional technologies

We are using different procedures for the study of somatic (tumor marker) and germline mutations, polymorphisms or for methylation and hydroxymethylation analysis

In relation to the type of libraries, we can generate them by amplicons or by enrichment systems, and for the study of mRNAs, miRNAs and other small RNAs, methylome, metagenome (rRNA or complete genome), etc
Seqplexing is Lexogen's reference laboratory in Spain thanks to Isogen Life Science

These studies can be performed from independent samples to thousands of samples, from single cell, waxed samples, swabs, FTAs, saliva, etc

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Seqplexing

Quantitative PCR in real time

Conventional technologies

We develop DNA or RNA quantification studies (including miRNAs) by quantitative PCR with LightCycler 480 II (Roche) alone or by multiplex. Specific designs for chimeric genes, bacteria, viruses, miRNAs, mRNAs, genotyping, etc

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